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Hughes ES , Bell JE , Simmonds P . 1997. Investigation of population diversity of human immunodeficiency virus type 1 in vivo by nucleotide sequencing and length polymorphism analysis of the V1/V2 hypervariable region of env. J Gen Virol , 78 ( Pt 11) (11), pp. 2871-2882. | Show Abstract | Read more

In this study we have analysed variability in the V1 and V2 regions of human immunodeficiency virus type 1 (HIV-1) proviral sequences amplified from lymphoid tissue, brain and other non-lymphoid tissue collected at autopsy from three HIV-1-infected individuals with giant cell encephalitis. We found no evidence for any tissue-specific grouping of variants in the V1/V2 regions, in contrast to previous comparisons of sequences in the V3 region, but consistent with the existence of evolutionarily distinct lineages previously observed in these study subjects by sequence comparisons of the p17gag gene. Examination of inferred amino acid sequences from V1 and V2 revealed no correlations between tissue origin with overall charge, length or number of glycosylation sites. Length polymorphism analysis is a rapid method to compare whole populations of HIV-1 variants within a sample, and provides information on the length and diversity of the V1 and V2 hypervariable regions. Based upon a comparison of 42 individuals with CD4 counts ranging from 802 to < 1 at time of death, we found no evidence for changes in the length of V2 with development of AIDS. Using the number of length variants in the V1 and V2 hypervariable region as a marker of the overall degree of variability within HIV populations, we found no evidence for an increase or a decrease in diversity between those with and without AIDS defining illness.

Cited:

24

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Haydon GH , Jarvis LM , Blair CS , Simmonds P , Harrison DJ , Hayes PC . 1997. Clinicopathological characteristics of subpopulations serum RT-PCR negative and serum RT-PCR positive for hepatitis C virus GUT , 41 pp. A126-A127.
Haydon GH , Jarvis LM , Blair CS , Simmonds P , Harrison DJ , Hayes PC . 1997. Clinicopathological characteristics of subpopulations serum RT-PCR negative and serum RT-PCR positive for hepatitis C virus (HCV) RNA. HEPATOLOGY , 26 (4), pp. 51-51.
Bouali MR , Khediri MF , Hmida J , Hila A , Davidson F , Simmonds P . 1997. Genotypes of hepatitis C virus: A new subtype 4. HEPATOLOGY , 26 (4), pp. 332-332.
Willson RA , Yap PL , Fischer SH , Ochs HD , Simmonds P . 1997. Long-term (7 yrs) interferon alfa maintenance therapy, with biochemical and virologic response, failed to prevent progression of chronic hepatitis C infection acquired through contaminated intravenous gamma globulin in a patient with common variable immune deficiency HEPATOLOGY , 26 (4), pp. 1181-1181.

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Hepatitis C virus (HCV) is transmitted through infected blood and blood products, but evidence of other routes of transmission is less clearly understood. In a study designed to examine human immunodeficiency virus (HIV) transmission, the prevalence of HCV has also been measured. Sixty-one couples were analysed, 30 in which partners were at risk through sexual contact alone, of whom 12 (40%) became infected with HIV and none with HCV. Thirty-one partners were exposed sexually and additionally through intravenous drug use. Of these, 16 (52%) became infected with HIV and 25 (80%) contracted HCV infection. These findings support the evidence of others that HCV is only rarely transmitted by sexual intercourse in heterosexual relationships and that HIV is not a co-factor for HCV transmission.

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How to identify the pest, the nest and the threat

Stinging insects such as various types of wasps, yellowjackets, hornets and bees, are common summertime pests and their stings can be more than just a painful nuisance.The National Pest Management Association reports that Karl Lagerfeld K/Ikonik Pins Free Shipping 2018 5lx1KQ5vGs
every year.Those with allergies to stings are most at risk, although anyone can be affected if a large number of stinging insects swarm and sting at once.

One way to protect yourself and your family from types of stinging insects like wasps and hornets this summer is to ensure your property is free from hives and nests. On a routine basis, walk around the exterior of your home, paying special attention to overhangs, eaves, the underside of porches and decks for nests. Also inspect shrubs, trees, sheds and other structures. If you do find a nest on your property, do not attempt to remove it on your own. The colony can become defensive and attack en masse.Instead, contact a licensed pest control professional who will be able to relocate or remove the hive in a safe manner.

Some stinging insects pose more serious threats than others. To determine the risk to your family, you will need to identify the type of insect, especially for wasps and yellowjackets. A trained pest professional will be able to properly identify a pest species and its threats, but you can also use this guide to help determine the species:

Types of Stinging Insects

Bumble Bees

Carpenter Bees

Honeybees

Baldfaced Hornets

European Hornets

Mud Daubers

Velvet Ants

Paper Wasp

Yellowjacket

Remember, it is not advised to attempt to remove a stinging insect nest on your own, and doing so can be extremely dangerous. Instead, work with a licensed pest professional to access your property and the nest, to identify the type of stinging insect (like wasps or other dangerous stingers), and to determine the best way to eliminate the threat to your family.

Threat:

NPMA Entomologist Dr. Michael Bentley spoke with Popular Science aboutants. Find out how bad ants will be this year, waysto keep them out and how to get rid of them.

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Simmonds P , Kurtz J , Tedder RS . 2002. Alternatives to nucleic acid testing in the blood transfusion service - Reply LANCET , 360 (9344), pp. 1519-1520. | American Tourister Joyride Spinner 55/20 Hand Luggage 55 cm 375 liters With Paypal Online Discounts Sale Online JL4qt
Zeytinoğlu A , Erensoy S , Abacioğlu H , Sayiner AA , Ozacar T , Başçi A , Kaplan H , Simmonds P , Bilgiç A . 2002. Nosocomial hepatitis C virus infection in a renal transplantation center. Clin Microbiol Infect , 8 (11), pp. 741-744. | Show Abstract | Read more

Nosocomial hepatitis C virus (HCV) infections were recorded in the renal transplantation unit of the university hospital. There were cases of acute HCV infection with aggressive clinical courses diagnosed from a positive HCV RNA test in the early post-transplantation period and which remained anti-HCV negative. Their anti-HCV seronegativity was attributed to them having acquired HCV under intense immunosuppressive therapy and suggested that the aggressive clinical course could be due to the deficient immune response resulting in an inability to limit viral replication. There were also donors diagnosed as having acute HCV infection in the early post-operative period. Genotyping and sequence analysis for HCV were performed on the isolates of eight of these patients who were consecutively transplanted and of three donors whose recipients were infected with HCV prior to transplantation, and who acquired acute HCV infection after transplantation. Of the eight recipients in the first group three were genotype 1a, three were genotype 1b, one was genotype 3a, and the last one was genotype 4 according to Simmond's classification. Of the three donor-recipient couples both the HCV isolates from one couple were genotyped as 1b and the phylogenetic analysis indicated that the patients were infected with a common variant of HCV, but the genotypes of HCV isolates from the other couples were different. Recipients were genotype 1b and the donors were genotype 1a in these couples. Genotype results of the first group and donor-recipient couples, and sequence analysis of genotype 1b and 1a isolates, showed that the source of infection was not a unique strain and there were multiple breaks in universal precautions while managing these patients.

Citation information in Europe Pubmed Central

Hepatitis C virus (HCV) genotype 1a and 3a partial NS5B gene segment sequences obtained from 154 HCV-infected injection drug users were studied to determine the extent to which HCV transmission occurs between injection drug user communities in London, Edinburgh, Glasgow (United Kingdom), Marseilles (France), and Melbourne. Phylogenetic relationships between sequences were analyzed by conventional methods and by a recently developed method that numerically scores the extent of sequence segregation between groups through calculation of association indices. The association indices revealed that none of the cities sampled support an HCV population that is completely isolated from that circulating in the other cities. Sequences from Melbourne were most isolated, whereas those from London were most dispersed. This suggests that HCV transmission between these cities occurs, with London playing a pivotal role. The degree of city-specific segregation of HCV subtype 1a sequences was linearly related to that of subtype 3a, indicating that these subtypes have spread through similar transmission networks.

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